大肠杆菌在自然环境中,饲料、饮水、动物的体表、孵化场、孵化器等各处普遍存在,是由大肠杆菌埃氏菌的某些致病性血清型菌株引起的疾病总称,也由一定血清型的致病性大肠杆菌及其毒素引起的一种肠道传染病。此次实验室里的老师需要用快速样品研磨仪来研磨菌类提取蛋白,比如大肠杆菌、芽孢杆菌等类。
实验地点:中南民族大学
实验材料和仪器:
样品:大肠杆菌
仪器:上海净信全自动样品研磨仪(JXFSTPRP-24L)
耗材:离心管,氧化锆珠
实验步骤:
1.准备2ml*24适配器,钢珠
2.将装好的样品规范放入JXFSTPRP-24研磨仪中
批量研磨样品,一般情况下,由于客户研磨的是菌类,组织偏小,研磨管内配比较为讲究,设置参数
3.得到如图所示研磨样品
实验过程、结果图示:
如上图为我们的实验材料:
测试中
注意:
1、每支离心管样品量一般不超过离心管体积的三分之二。
2、样品尽量对称放置,以保证研磨时的受力平衡。
3、珠子和液体比例1:1或者1:2效果最好,珠子比例1:1放入
仪器介绍:
上海净信®的JXFSTPRP-L®系列组织研磨仪(专利产品,仿冒必究!ZL2019301918692)是一种特殊的、快速的、高效率的、多试管的一致系统。它能将任何来源(包括土壤、植物和动物的组织/器官、细菌、酵母、真菌、孢子、古生物标本等)的原始DNA、RNA和蛋白质进行提取和纯化。将样品和研磨球放入研磨仪内(配置研磨罐或离心管/适配器),在高频摆动作用下,研磨球在研磨仪内来回高速撞击及摩擦,在非常短的时间内便可完成样品的研磨、粉碎、混合及细胞破壁。
性能特点:
1.稳定性好:采用三维一体8字形振荡方式,研磨更充分,稳定性更好;
2.效率高:1分钟内完成2×24、2×48、4×96个样品的研磨;
3.重复性好:同一组织样本设定相同程序,获得相同的研磨效果;
4.操作简便:内置程序控制器,可对研磨时间、转子的振动频率等参数进行设置;
5.安全性高:具备安全罩及安全锁;
6.无交叉污染:在研磨过程中处于全封闭状态,避免交叉污染;
7.低噪音:仪器运行过程中,噪音小于55dB,不会对其它实验或仪器产生干扰。
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