Model：81156 81151 80050
Brand：ibidi
Specifications ：60 pieces/box
Introduction：A 35 mm imaging dish with a polymer coverslip bottom for high-end microscopy and cell-based assays In this microscopy dish, the cells are imaged on a No. 1.5 ibidi Polymer Coverslip bottom with the highest optical quality and optimal cell adherence
Applications
Cultivation and high-resolution imaging of cells
Immunofluorescence staining
Widefield and confocal fluorescence microscopy of living and fixed cells
Live cell imaging over extended time periods
Transfection assays
Differential interference contrast (DIC) when using a DIC Lid
Cell location and counting when using the gridded version
Specifications
| Ø µ-Dish | 35 mm |
| Volume | 2 ml |
| Growth area | 3.5 cm2 |
| Coating area using 400 µl | 4.1 cm2 |
| Ø observation area | 21 mm |
| Height with / without lid | 14/12 mm |
| Bottom: ibidi Polymer Coverslip | |
Technical Features
Standard format imaging dish with a 35 mm diameter for tissue culture
ibiTreat (tissue culture-treated) surface for optimal cell adhesion and cell growth
High walls with a standard height for easy handling; low wall version available: μ-Dish 35 mm, low
Lid with locking feature for minimal evaporation
Rim for easy opening
No autofluorescence
Biocompatible plastic material—no glue, no leaking
Available as a Bulk Pack with 400 individually packed μ-Dishes per box
Available with a #1.5H Glass Coverslip Bottom: μ-Dish 35 mm, high Glass Bottom, specifically suitable for TIRF and single molecule applications
Available with a 500 μm grid for cell counting: μ-Dish 35 mm, high Grid-500
Available with a non-adhesive Bioinert surface: μ-Dish 35 mm, high Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells
The Principle of the μ-Dish 35 mm, high
The Coverslip Bottom
The μ-Dish 35 mm, high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.
As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish 35 mm with a #1.5 glass coverslip bottom.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
Lid with Locking Feature for Minimized Evaporation
All ibidi μ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.
TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).
Experimental Examples：
ibidi µ-Dish Height Variations
µ-Dish 35 mm, high
High walls for all standard cell culture applications
μ-Dish 35 mm, low
Low walls that provide greater cell access, which is useful for micromanipulation
Triple immunofluorescence of bovine endothelial cells.
Red: mitochondria, stained with MitoTracker? Red CMXRos;
Green: F-actin, stained with Alexa Fluor? 488 Phalloidin;
Blue: nuclei, stained with DAPI.
Comparison of the ibidi Dishes 35 mm
Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials here.
