1)时间:2014/3/10 注:部分请与厂家工程师联系
2)样品:猪肌肉组织
3)仪器:主机,Tissuelyser动物组织研磨器(上海净信实业有限公司生产)
4)配件:离心管和净信研磨珠
4)步骤:
a.取肌肉组织样品,均匀地剪切成少量样品块,分别放入若干研磨离心管中。
b.在每个试管中放入几颗净信研磨珠和一些PBS裂解液,分别盖紧管盖后,再置入研磨仪的适配器中,将适配器固定在研磨仪中。
c.将Tissuelyser动物组织研磨器的时间和频率参数设置好后,就可以启动研磨仪了。
d.研磨完全停止后,拿出研磨离心管,旋开管盖,取出研磨好的肌肉组织样品。
5)结果:
研磨后的猪肌肉组织成很均细的泥浆状,说明用Tissuelyser动物组织研磨器研磨猪肌肉组织效果良好。
上海净信的全自动样品快速研磨仪处理与手工液氮研磨处理后提取 FT 的 DNA、RNA 在浓度、纯度上均无显著性差异 。但手工研磨有较大的样本损失率,对于DNA提取浓度较低的样品来说,自动研磨仪可以做到样品零损失,在DNA提取上有着明显的优势,且手工处理的过程复杂、易交叉污染,操作所用时间等方面均不及上海净信的全自动样品快速研磨仪,因此自动研磨仪对于DNA提取工作相对于手工研磨处理有很大优势。
Previous:自动研磨仪在动物样品处理中的应用
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