课题组介绍:张天真,求是特聘教授,博导,研究方向:作物遗传育种。主要开展棉花结构基因组、重要性状基因克隆与功能验证、多倍体遗传与分子进化和生物信息学等方面研究。
实验目的:研磨棉花幼苗枝干提取DNA及RNA
实验地点:浙江大学农业与生物技术学院
实验材料和器具:
样品:棉花幼苗枝干
仪器:全自动低温冷冻研磨仪(上海净信JXFSTPRP-CLN)、钢罐一套
实验步骤:
1、将棉花幼苗枝干剪成2-5mm长短的样品;
2、将处理好的样品加入钢罐中;
3、加入配套的研磨珠子一颗,放入液氮种预冷几分钟;
4、将冷冻过的钢罐样品装入净信研磨仪中;
4、设定运行参数;
5、启动仪器至研磨程序结束,可得到如图所示样品。
实验结果:
仪器介绍:
净信上海净信JXFSTPRP-CLN冷冻研磨仪是一种快速高效、多试管的一致系统。它能将任何来源(包括土壤、植物和动物的组织/器官、细菌、酵母、真菌、孢子、古生物标本等)的原始DNA、RNA和蛋白质进行提取和纯化。广泛应用于生命科学、医药、生化、环境、地质、农业、林业、质检、材料等领域。
JXFSTPRP-CLN产品原理:
将样品和研磨球放入研磨仪内(配置研磨罐或离心管/适配器),在高频摆动作用下,研磨球在研磨仪内来回高速撞击及摩擦,在非常短的时间内便可完成样品的研磨、粉碎、混合及细胞破壁。
性能特点:
1.不破坏样品成分:研磨温度可自定义调节,能够有效抑制核酸降解,保留蛋白质活性;
2.制冷可调:-50至37度
3.高通量处理量:一次处理多达192个样品
4.操作简便:内置程序控制器,可对参数进行设置;
5.安全性高:具备安全罩及安全锁,无需涉及液氮操作,安全性高;
6.无交叉污染:在研磨过程中处于全封闭状态,避免交叉污染;
7.低噪音:仪器运行过程中,噪音小于55dB,不会对其它实验或仪器产生干扰。
8.无易损件设计,电磁锁定保护,不锈钢内腔,清洁消毒方便
9.重复性稳定:相同研磨程序获得相同效果
10.程序可存储:可存储10组自定义研磨程序
11.研磨效果好:出料粒度5um
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