实验目的:关于家禽(牛、羊)肌肉中DNA提取
实验地点: 浙江省农业科学院畜牧兽医研究所
实验材料和器具
样品:羊的后退肌肉
仪器:全自动样品快速研磨仪(上海净信Ts-24)、24位金属适配器
耗材:XXmm钢珠、XXmm钢珠、裂解液
实验步骤
1、将整块样品从冰箱中取出,放入液氮中冷冻及分钟;
2、冷冻过后的样品用先用硬物敲碎预处理;
3、将适配器放入液氮中预冷;
4、设定好运行参数;
5、离心管内加入钢珠几颗,加入适量样品和裂解液;
6、迅速将装有样品的离心管放入预冷的适配器中
7、启动仪器至研磨程序结束,可得到研磨后样品;
实验结果
Previous:多样品组织研磨机Tissuelyser-24研磨指甲-上海净信
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