实验目的
研磨果蝇,更有效快速的提取核酸蛋白。
实验地点:国家蛋白质(上海)中心C座319
实验材料和器具
样品:果蝇60只,大概60mg
仪器:全自动样品快速研磨仪(上海净信,JXFSTPRP-24)
耗材:专用研磨管(上海净信,2ml),研磨珠(上海净信)
试剂:液氮若干,PBS
实验步骤
1.准备好2ml(样品量大的话,可选5ml规格)不锈钢研磨管,将研磨管和研磨球清洗干净,并灭菌灭酶;
2.将需要研磨的组织样品放入准备好的研磨管中,加入少许研磨珠。盖好盖子;
3.取出已经冷冻的适配器,把装好样品研磨管插入适配器中;
4.将适配器装入净信研磨仪中;
5.设定好参数,启动仪器至研磨程序结束。
实验结果
Previous:冷冻研磨仪研磨破碎大肠杆菌——上海净信
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