大肠埃希氏菌(Escherichia coli)通常被称为大肠杆菌,是Escherich在1885年发现的,在相当长的一段时间内,一直被当作正常肠道菌群的组成部分,认为是非致病菌。直到20世纪中叶,才认识到一些特殊血清型的大肠杆菌对人和动物有病原性,尤其对婴儿和幼畜(禽),常引起严重腹泻和败血症,它是一种普通的原核生物,根据不同的生物学特性将致病性大肠杆菌分为6类:肠致病性大肠杆菌(EPEC)、肠产毒性大肠杆菌(ETEC)、肠侵袭性大肠杆菌(EIEC)、肠出血性大肠杆菌(EHEC)、肠黏附性大肠杆菌(EAEC)和弥散粘附性大肠杆菌(DAEC)。大肠杆菌属于革兰氏阴性细菌(G-)。
高密度发酵是现代发酵工程的一顶新技术,能显著提高大肠杆菌菌体和基因工程产品的产量。通过用净信冷冻研磨仪,能快速,有效的对大肠杆菌进行破壁处理,能更好的进行下一步研究。
实验步骤
步骤1:把发酵好的样品放到离心管中,加入适量的研磨珠;
步骤2 :将样本放入冷冻适配器中,放入净信冷冻研磨仪上;
步骤3:设置参数:研磨频率X和研磨时间;开启制冷模式,使样品研磨的环境在特定的温度下进行,时间X分钟就对大肠杆菌进行完全破壁。
步骤4:从净信冷冻研磨仪上取出离心管,放到离心机离心一下,便可轻松提取样品进行进样处理。
需了解具体步骤,请致电厂家!
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