实验目的:研磨水稻种子更有效快速的提取核酸。
实验地点:中国科学院植物研究所
实验材料和器具:
样品:水稻种子
仪器:全自动快速研磨仪(上海净信,JXFSTPRP-64)
配件:不锈钢研磨罐(上海净信),研磨珠(上海净信)
研磨条件:干磨液氮预冷处理
实验步骤
1.将净信专用研磨罐和研磨球用乙醇清洗干净,并灭菌灭酶;
2.取适量需要研磨的水稻种子装入研磨罐中,加入研磨珠,盖好盖子;
3.取液氮放在泡沫箱中,将装好水稻种子的研磨罐置于液氮冷冻几分钟。
4.将研磨罐装入研磨仪。
4.设定好参数,启动仪器至研磨程序结束
需了解具体步骤,请致电厂家
实验研磨结果
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