实验目的:研磨肌肉组织,更有效快速的提取核酸蛋白。
实验地点:清华大学
实验材料和器具
样品:小鼠肌肉组织
仪器:高通量组织研磨仪(上海净信,TISSUELYSER-24)
耗材:专用研磨管(上海净信),研磨珠(上海净信)
试剂:PBS缓冲液
实验步骤
1.将净信专用研磨管和研磨球用乙醇清洗干净,并灭菌灭酶;
2.将需要研磨的肌肉组织样品剪成小块备用。
3.放入准备好的肌肉组织到研磨管中,加入研磨珠,盖好盖子;
4.将预冷好金属适配器装入净信研磨仪,将研磨管装入适配器。固定好。
4.设定好参数,启动仪器至研磨程序结束。
实验前后对比
Previous:全自动样品快速研磨仪研磨肾组织-上海净信
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