实验目的:研磨肾组织,更有效快速的提取核酸蛋白。
实验地点:浙江大学紫荆港校区医学院C座214实验室
实验材料和器具:
样品:小鼠肾组织
仪器:高通量组织研磨仪(上海净信,JXFSTPRP-24)
耗材:专用研磨管(上海净信),研磨珠(上海净信)
试剂:PBS缓冲液
实验步骤
1.准备好研磨管,将研磨管和研磨球清洗干净,并灭菌灭酶;
2.将需要研磨的肾组织样品剪一小块,放入准备好的研磨管中,加入研磨珠,盖好盖子;放到适配器中,适配器可以事先放在冰箱先预冷,在干磨过程中相对的控制温度。
3.将适配器装入净信研磨仪中;
4.设定好参数,启动仪器至研磨程序结束。
实验结果
Previous:多样品组织研磨仪研磨破碎皮肤、动植物及各材料标本实验-上海净信
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