动物组织RNA的提取,采用传统研磨方法效果不佳,难以达到后续实验要求,如:加入液氮后于研磨钵中手工研磨、快速匀浆器等。只有对组织进行充分研磨才能保证RNA及蛋白的有效提取,进而为荧光定量PCR、Western-blot等实验的进一步开展提供可靠的实验材料。证实上海净信的组织研磨破碎仪可有效处理该类组织,能够达到实验的要求。
动物组织标本样
实验步骤
步骤1:把采集的临床标本剪切成小块;
步骤2 :将样本放入研磨管中,再转移至净信研磨仪适配器;
步骤3:设置参数:研磨频率,时间,打开研磨管可见样本已被充分研磨成黏稠状;
步骤4:收集样本冻存或进行后续实验。
实验后续结果
提取的RNA及蛋白浓度较手动研磨方式有极大提高,可完全满足后续实验需求。
Previous:全自动组织研磨机研磨大鼠脚掌实验-上海净信
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