实验目的:研磨破碎大鼠脚掌
实验原理
大鼠脚掌因为有皮肤及毛发加上含有一些筋,韧性很强,所以难以破碎,需配合液氮研磨才能得到较好的研磨效果。
实验材料和器具:
样品:大鼠脚掌200毫克
仪器:高通量组织研磨仪(上海净信,JXFSTPRP-24)
耗材:研磨钢罐(上海净信),研磨珠(上海净信)
试剂:液氮若干
实验步骤:
1.准备好研磨管,将研磨管和研磨球清洗干净,并灭菌灭酶;
2.将需要研磨的组织样品放入准备好的研磨管中,加入研磨珠。盖好盖子;
3.将装有组织样品的研磨管放入液氮中,冷冻至不沸腾为止;
4.取出已经冷冻的适配器,把冷冻好的不锈钢研磨管取出插入适配器中;
5.将适配器装入净信研磨仪中;
6.设定好参数,启动仪器至研磨程序结束,可得到粉末状样品。
实验结果
Previous:全自动组织研磨机破碎酵母细胞实验-上海净信
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