实验背景
大、小鼠皮肤提蛋白目前是各高校动物实验室的一项难题,目前客户用匀浆器手动研磨方法操作麻烦,温度控制难度大,蛋白容易在研磨过程中因瞬时温度和研磨颗粒较大,无法做出好的实验效果。上海净信研磨仪根据客户要求提供技术支持,解决华中科技大学生科院某实验室技术难题。
某生物实验交流网站帖子询问WB解决方案,无准确回答解决方案。
实验解决方案:
1.仪器配置:上海净信多样品组织研磨仪一台,模块一套。
研磨仪主机/5ml不锈钢罐适配器
2.实验器材需求:适量大小保温瓶一个,眼科剪,镊子,钥勺,后续配套离心管、裂解液。
皮肤去脂肪层铺展开效果/ 取材,准备放入适配器液氮冷冻
将皮肤放入5ml不锈钢罐,剪碎至一定程度,放入钢珠,然后一同放入保温瓶中冷冻至不沸腾,大概3分钟左右将适配器一同快速装入研磨机中,调好频率和时间研磨。研磨完成后将组织转入EP管中用裂解液溶解。
皮肤研磨后效果图/加入裂解液后效果图
研磨完成后进行体蛋白提取及后续实验,此方法最小通量为24个2mlEP管。大大提高了实验人员的工作效率,并且研磨时间短并且均衡,能控制好全程温度在-80℃以下。
总结:上海净信研磨仪对皮肤及动、植物或各种材料标本有全套解决方案,高通量地解决实验重难点问题,让实验更加轻松快捷,省去大量人力物力,深受各高校喜爱。
Previous:多样品组织研磨仪研磨猪皮实验-上海净信
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