近年来,随着生命科学研究的发展,尤其是在完成拟南芥 (Arabidopsis thaliana) 和水稻 (Oryza sativa) 等植物的基因组测序后,植物生物学发生了翻天覆地的变化。人们已经把目光从基因的测序转移到了基因的功能研究。在研究 DNA 的基因组学、mRNA 的转录组学及蛋白质的蛋白组学后,接踵而来的是研究代谢物的代谢组学 (Hall et al.,2002)。代谢组学的概念来源于代谢组,代谢组是指某一生物或细胞在一特定生理时期内所有的低分子量代谢产物,代谢组学则是对某一生物或细胞在一特定生理时期内所有低分子量代谢产物同时进行定性和定量分析的一门新学科 (Goodacre,2004)。它是以组群指标分析为基础,以高通量检测和数据处理为手段,以信息建模与系统整合为目标的系统生物学的一个分支。
实验步骤:
步骤1:将采集的各种植物组织(根茎叶,根瘤菌等)装入研磨管,加入研磨珠。放入液氮中进行低温处理。
步骤2:将低温处理过的样品放入净信研磨仪,设置频率,研磨完成后可以得到粉末状的样品
步骤3:从净信研磨仪中取出样品,将样品溶解做后续实验
实验结果
研磨效果
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