实验目的
对拟南芥或水稻穗进行研磨破碎,进而提取其DNA。
实验原理
通过研磨珠的撞击,充分拟南芥或水稻穗细胞,使其内容物流出,进而提取目标成分。
实验材料和器具
样品:拟南芥或水稻穗
仪器:全自动样品冷冻研磨仪(上海净信,Tissuelyser-48)
耗材:离心管,不锈钢研磨珠(上海净信)
试剂:TPS溶液(100mM Tris-Cl (pH 8.0),10mM EDTA (pH 8.0),1M KCl)
实验步骤
需了解具体步骤,请致电厂家
实验注意事项
1.-20摄氏度下该方法的DNA可以保存4个月以上,4°大致可以放2-3周。
Previous:tissuelyser组织研磨机对酵母菌研磨破壁方法
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