实验目的
研磨破碎多酚含量高的植物(木本植物),后续可提取其核酸或蛋白。
实验原理
充分磨碎研磨破碎多酚含量高的植物,再用相关试剂提取其总RNA。
实验材料和器具
样品:银杏叶子
仪器:高通量组织研磨仪(上海净信,Tissuelyser-24)
耗材:离心管,研磨珠
实验步骤
需了解具体步骤,请致电厂家
实验注意事项
如果需要提取的样品比较多,研磨好的样品在后续的提取中,需要转移到大一点的epp管中,研磨时可以加入较少的抽提液,研磨完毕按照样品的体积去提取液补充抽提液,进行后续的操作。
Previous:高通量组织研磨仪针对检测心肺组织的药物代谢的研磨方法
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