实验目的
通过提取药材的DNA,进行DNA条形码分子对照,进而鉴定中药。实验原理
通过对照药材自身独有的DNA条形码记录,可以准确鉴定出中药材品种。用DNA条形码分子法鉴定中药是药典中一项重要的鉴定方法。
实验材料和器具
样品:甘草,陈皮,阿胶饮片
仪器:高通量组织研磨仪(上海净信,Tissuelyser-24)
耗材:不锈钢研磨罐,研磨珠(上海净信)
实验步骤
需了解具体步骤,请致电厂家
注意事项
1. 研磨罐和研磨珠需充分清洗干净并烘干备用;若需提取药材的RNA,则研磨罐和破碎珠要确保灭酶;
2. 每个研磨罐大约可研磨适量大小的药材量(具体根据样品本身的紧密度会有变动,一般样品体积不超过研磨罐容积的三分之二);
3. 如果放入的样品没有脱水或者是水分含量较大的药草类,需用液氮预冷,具体操作是:把装好样品和研磨珠的研磨罐浸入液氮中,直至液氮不沸腾,再迅速将研磨罐放入研磨仪中,设置参数单位振频,间歇几十秒后启动研磨仪,就能得到较好的研磨效果。较坚硬的样品无需液氮冷冻,如阿胶、鹿茸等。
研磨效果
Previous:高通量组织研磨仪对经济作物水稻、小麦种子的研磨
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