实验目的
农产品及种子检验,经常需要对将植物种子进行研磨以获得DNA、RNA或蛋白质等生物分子,从而进行后续的分析和检测。随着实验室科研进度的日益加快,手动研磨已无法满足科研工作的需要。
本实验通过高通量研磨破碎经济作物种子,进而提取其核酸/蛋白质进行种子检验和分析。
实验材料和器具
样品:水稻,小麦种子
仪器:多样品组织研磨仪(上海净信,Tissuelyser-24)
耗材:离心管,研磨珠(上海净信)
实验步骤
需了解具体步骤,请致电厂家
注意事项
1. 离心管和研磨珠需充分清洗干净并烘干备用;若需提取RNA,则研磨罐和研磨珠要确保灭酶;
2. 每个离心管研磨样品量一般遵循原则:样品体积不超过离心管容积的三分之一。
研磨结果
Previous:高通量组织研磨仪对动物器官组织的研磨
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