1)时间:2014/9/11
2)样品:枸杞
3)仪器:主机,Tissuelyser动物组织研磨器(上海净信实业有限公司生产)
4)配件:离心管和净信研磨珠珠
4)步骤:
A.将提前准备好的枸杞分装在离心管中。
b.每个试管中放入研磨珠和PBS裂解液,分别盖紧管盖后,再置入研磨适配器中,将适配器固定在研磨仪中。
c.将Tissuelyser植物组织研磨仪的参数按要求设置好。启动研磨器。
d.研磨完全停止后,拿出研磨好的离心管,打开管盖,取出研磨好的枸杞组织样品。
5)结果:
研磨后的枸杞组织成很均细的泥浆状,说明用Tissuelyser植物组织研磨仪研磨枸杞组织效果非常好,可以用于下一步实验。
上海净信的全自动样品快速研磨仪处理与手工液氮研磨处理后提取 FT 的 DNA、RNA 在浓度、纯度上均无显著性差异 。但手工研磨有较大的样本损失率,对于DNA提取浓度较低的样品来说,自动研磨仪可以做到样品零损失,在DNA提取上有着明显的优势,且手工处理的过程复杂、易交叉污染,操作所用时间等方面均不及上海净信的全自动样品快速研磨仪,因此自动研磨仪对于DNA提取工作相对于手工研磨处理有很大优势。
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