1)时间:2014/03/10 注:部分请与厂家工程师联系
2)样品:水稻叶片
3)仪器:主机,Tissuelyser植物组织研磨器(上海净信实业有限公司生产)
4)配件:离心管和净信研磨珠
5)步骤:
A.取水稻叶片组织,用剪刀剪成若干段,分别放入离心管中。
b.每个离心管中放入几颗净信研磨珠和7微量的裂解液,分别将离心管盖盖好后,再置入研磨适配器中,将适配器固定在研磨仪中。
c.设置Tissuelyser植物组织研磨仪的研磨时间和频率的参数。启动研磨器。
d.研磨完全停止后,拿出适配器上的离心管,打开管盖,取出研磨好的水稻叶片样品。
6)结果:
由图可以清晰的看出,研磨后的水稻叶片组织很均细的匀浆状,说明用Tissuelyser植物组织研磨仪玫瑰花瓣效果非常良好,可以用于后续转基因检测工作。
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